Effect of Er: YAG Laser Irradiation on Bone Metabolism-Related Factors Using Cultured Human Osteoblasts

Introduction: A variety of laser treatments have been applied in numerous medical fields. In dentistry, are used for caries, root canals, and periodontal disease, as well surgical resection. Numerous reports recently published on the use lasers bone regeneration. If irradiation is found to promote activation metabolism, it might also be effective treatment, peri-implantitis, Therefore, present vitro study aimed elucidate mechanisms underlying effects erbium-doped yttrium aluminum garnet (Er: YAG) using osteoblast-like cells. Methods: Osteoblast-like Saos 2 cells (5.0×104 cells) were seeded 24-well plates. 24 hours after being seeded, subjected 0.3 W, 0.6 2.0 W Er: YAG then allowed recover 48 hours. The expression levels metabolism-related factors alkaline phosphatase (ALP), sialoprotein (BSP), osteoprotegerin (OPG) evaluated reverse transcription–quantitative polymerase chain reaction western blot analyses. Results: at showed normal growth. When control groups compared hours, increases observed ALP, BSP, OPG gene protein group. Similar results obtained analysis. Conclusion: These findings suggest that activating metabolism factors.